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Chitin and Chitinases: Biomedical And Environmental Applications of Chitin and its Derivatives

Abstract:

Disposal of chitin wastes from crustacean shell can cause environmental and health hazards. Chitin is a well known abundant natural polymer extracted after deproteinization and demineralization of the shell wastes of shrimp, crab, lobster, and krill. Extraction of chitin and its derivatives from waste material is one of the alternative ways to turn the waste into useful products. Chitinases are enzymes that degrade chitin. Chitinases contribute to the generation of carbon and nitrogen in the ecosystem. Chitin and chitinolytic enzymes are gaining importance for their biotechnological applications. The presence of surface charge and multiple functional groups make chitin as a beneficial natural polymer. Due to the reactive functional groups chitin can be used for the preparation of a spectrum of chitin derivatives such as chitosan, alkyl chitin, sulfated chitin, dibutyryl chitin and carboxymethyl chitin for specific applications in different areas. The present rev...

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Physico-Chemical and Bacteriological Quality of Water from Boreholes in Otuoke Community, Bayelsa State, Nigeria

Abstract:

The concern over exposure to drinking water contaminated and the resultant adverse effect on human health has prompted several studies evaluating the quality of drinking water sources. This study was carried out to determine the bacteriological and Physico-chemical qualities of commercial borehole water within Otuoke community in Bayelsa state Nigeria. Ten (10) water samples were collected from the various locations designated as (A-J). The Physico-chemical parameters were determined by using the photometric technique through the Colour Q photometer and the bacteriological analysis was determined by using aerobic plate method. The Physico-chemical parameters of the water samples analysed were within the acceptable limit of WHO standard of drinking water quality except for Bromine and cyanuric acid which ranged from 1.6±0.1 to 2.7±0.1 and 2±1 to 9±1 respectively. The Result of total bacteria count obtained from the borehole water samples ranged from 1.1 x 10 Read Article

Hydrolysis of Cellulose by Soluble Clostridium Thermocellum and Acidothermus Cellulolyticus Cellulases

Abstract:

The goal of this work was to clone, express, characterize and assemble a set of soluble thermostablecellulases capable of significantly degrading cellulose. We successfully cloned, expressed, and purified eleven Clostridium thermocellum (Cthe) cellulases and eight Acidothermuscellulolyticus(Acel) cellulases. The performance of the nineteen enzymes was evaluated on crystalline (filter paper) and amorphous (PASC) cellulose. Hydrolysis products generated from these two substrates were converted to glucose using beta-glucosidase and the glucose formed was determined enzymatically. Ten of the eleven Cthe enzymes were highly active on amorphous cellulose. The individual enzymes all produced <10% reducing sugar equivalents from filter paper. Combinations of Cthe cellulases gave higher conversions, with the combination of CelE, CelI, CelG, and CelK converting 34% of the crystalline ce...

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The Effect of Laser Irradiation on Reparative Osteogenesis

Abstract:

Introduction:

The use of non-medicinal facilities for correcting processes in various pathological conditions is one of the most urgent problems of modern medicine.

Purpose of the Work:

To study the effect of low-intensity infrared laser radiation on reparative bone formation and angiogenesis in bone regeneration which is formed in treatment of fractures under conditions of transosseous osteosynthesis.

Material and Methods:

A tibia fracture was modeled experimentally in rats in the control and experimental groups. Reposition and fixation of fragments were performed. The fracture zone in the experimental group animals was exposed to the impact of pulsed infrared laser irradiation of low intensity. Animals from the control gr...

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Current Status and Future Perspectives of Gene Therapy for Heart Failure

Abstract:

Heart failure(HF) is a disease with high morbility and mortality. The benefits of current pharmacological and device therapy for survival outcomes of patients with HF are limited. Gene therapy represents a novel promising strategy in treating HF, as it can theoretically normalize the aberrantly expressed genes and their regulatory mechanisms permanently. However, the translation of gene therapy for HF from bench to bedside has been less successful. There are many challenges ahead for gene therapy, especially in the areas of selection of the optimal targets, the needs for developing delivery systems and the improvement in design of clinical trials. In this review, we summarize the most promising gene targets which have been used in experimental and clinical studies for treating HF, highlighting the results from several clinical trials. We also review the latest development in gene therapy vectors and delivery methods, aiming to provide directions for future studies...

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Intriguing Humans and Primates chromosomes 4

Abstract:

The global analysis of 3 human genomes of increasing levels of evolution (neanderthal / sapiens build34 of 2003 / sapiens hg38 of 2013) reveals 2 levels of numerical constraints controlling, structuring and optimizing the DNA sequences of these genomes.

A global constraint - which we will call "HGO" for "Human Genome Optimum" - optimizes the genome at its global scale of 3.5 billion base pairs. This same operator when applied to each of the 24 individual chromosomes reveals a hierarchical structure of these 24 chromosomes according to a numerical spectrum of amplitude ½ Phi extending from chromosome 4 to chromosome 19. This first level of comparison reveals a very Great analogy between these 3 genomes.

Then we introduce a global analysis method of roughness or fractal texture of the DNA sequences at the level of each chromosome. After having demonstrated that the chromosome4 seems to play a privileged role in the human genome, radica...

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Human Proteome Project and Current Bioinformatics Status in Disease Diagnosis and Treatment

Abstract:

Human proteome project was revolutionized about 40 years ago with purpose of summarizing whole proteomic data at one place. It was launched after human genome project to map and observe all proteins. The goal related proteomic study is to draft the entire human proteome in disease diagnosis by using bioinformatics tools. Pillars of human proteome project provide different databases related to proteins at transcriptional and translational level. Human proteome organization(HUPO) published biology disease HUPO whose aim is to measure protein and proteome by life and processes related to human diseases. Different human organ like plasma, liver, brain and diabetic base project are used to characterize human disease and health. Major data resources accumulated in databases like peptides Atlas, GPMDB and neXtProt for proteins. Matrices of human proteome project identify and characterize the protein products as Post translational modification (PTM), splice various isofor...

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Renal Function in Patients with Acute Myocardial Infarction: A Current Perspective.

Abstract:

A clinical perspective on renal function in acute myocardial infarction reviews pathophysiology, prognostic markers, and management implications, including contrast nephropathy prevention and hemodynamic support.

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Management of Arthrofibrosis of the Knee after an Arthroscopic Meniscectomy with Paralytic Shellfish Poisoning Toxin. Case Report.

Abstract:

Objective:

The purpose of this study is to evaluate the use of paralytic shellfish poisoning toxin in a patient with arthrofibrosis of the knee. Arthrofibrosis is a common complication of knee surgery that tends to manifest itself as a limitation of the musculoskeletal ranges. Paralytic shellfish poisoning toxin has been proposed as an alternative treatment for painful musculoskeletal pathologies.

Subjects:

Use of paralytic shellfish poisoning toxin in arthroscopic menisectomy. Case report.

Methods:

Three doses of paralytic shellfish poisoning toxin were administered in an intra-articular manner on different days. Functionality, musculoskeletal ranges, pain at rest and pain during motion were evaluated.

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Maxillofacial Trauma and Psychological Stress

Abstract:

A review links maxillofacial trauma to psychological stress responses. It discusses screening, early interventions, and multidisciplinary care to improve recovery.

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